What makes chloroplast appear completely different from other cell
organelles?
It was not
until 1954, that Daniel I. Arnon was able to break up plant cells so gently that whole
chloroplasts could be obtained that could carry through photosynthesis.
It has been found that the chloroplast is
a membranous structure, consisting of 3
membranes.
The third layer forms stacked
sack like structures called as grana. It is
believed to be a site for trapping of solar
energy. The intermediary fluid filled portion
is called as stroma.
It is believed to be
responsible for enzymatic reactions leading
to the synthesis of glucose, which in turn
join together to form starch.
Substances found in chloroplast which capture sunlight are called
photosynthetic pigments. There are several types of photosynthetic
pigments involved in the process to produce organic molecules like
glucose in plants.
Chlorophyll is such a pigment which contain one atom of magnesium.
It is similar in structure to the haem of haemoglobin.
(The iron containing
red pigment that transports oxygen in blood.)
Two major kinds of
chlorophylls are associated with thylakoid membranes. Chlorophyll ‘a’ is
blue-green in colour and chlorophyll ‘b’ is yellow-green colour.
Around
250 to 400 pigment molecules are grouped as light harvesting complex or
photosynthetic unit in each thylakoid. Such innumerable units function
together in chloroplasts of green plants in the process of photosynthesis.
During photosynthesis several events occur in the chloroplast some
of them are:
1. Conversion of light energy to chemical energy
2. Splitting of water molecule (photolysis of water)
3. Reduction of carbondioxide to carbohydrates
Light is required to initiate several events while several may continue
even in absence of it. That would mean, once light energy has been captured
it can help reactions to continue even in the dark. Light dependent events