form oxygen they have to get energy to make it possible. Where did the energy come from?

A Dutch scientist, Jan Ingenhousz (1730-1799), found the answer. He studied the way in which plants form oxygen. In 1779, he noticed that it happened only in the presence of light.

In an experiment with the aquatic plant, Hydrilla, he observed that in bright sunlight, small bubbles formed around the green parts while in the dark they did not form. He also found that the gas present in the bubbles was oxygen.

It was further confirmed when Engelman in the early 20th century ingeniously detected the point of maximum rate of photosynthesis. He used a strand of algae and exposed it to different colours of light

(the colours that we see in a rainbow) Then he used oxygen sensitive bacteria and found that they crowd around areas illuminated with red and blue rays of light.

This led to more studies on effect of light on photosynthesis, the role of different coloured compounds called pigments in plants and the utilization of light energy.

Lab Activity

Oxygen is produced during photosynthesis in the presence of light
Arrange the apparatus as shown in the figure.

Make two identical sets.
Place some water plant like Elodea or Hydrilla in a short stemmed funnel and keep it in a beaker.

Then pour the water in the beaker. Invert a test-tube full of water over the stem of the funnel. Ensure that the level of water in the beaker is above the level of stem of the inverted funnel.

Place one apparatus in the sun and the other in the dark for at least 2-3 hours.

You would see that in place of water there is air that fills in the set up kept in sun.

It is actually a gas that will be collected in the test-tube. Observe the other set up kept in dark.
Is there any difference in the amount of gas collected?
Test the gas in the test-tube by inserting a glowing match stick or incense stick which would burst into flames.

This shows the presence of oxygen.

What precautions do you need while removing test tube from the beaker.

Discuss with your teacher.



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